Internship in Software Development - II

BDG LifeSciences' Research Project Training Program is a one-of-a-kind initiative designed to strengthen your profile and enhance career opportunities, whether for jobs or higher studies, by offering the chance to work on novel research projects using the latest technologies in bioinformatics. Since 2010, this program has addressed the critical need for high-quality publications by combining innovative teaching methods with practical applications. Conducted entirely online, it provides participants with the flexibility to choose session timings while saving on travel, accommodation, and food expenses. With over 88 research projects successfully completed and published at the international level, this program is ideal as a major or thesis project for final-year students or for those looking to advance their profiles.

Applications are now open for 5 SEATS in our 93 novel research project entitled "Integrative Single-Cell Transcriptomics and CRISPR-Cas9 Perturbation Modeling for Regulatory Network Mapping in the Tumour Microenvironment". 

Introduction of the Project

The CRISPR-Driven Single-Cell Research Project by BDG LifeSciences is an advanced, structured, research-intensive initiative designed to decode the complexities of the tumour microenvironment using CRISPR-Cas9 perturbation analysis and single-cell transcriptomics.

Modern cancer biology demands more than theoretical knowledge. It requires systems-level thinking, computational depth, and the ability to interpret gene regulatory rewiring at cellular resolution. This project integrates CRISPR gene editing, bioinformatics, genomics, and immunogenomics into a unified research workflow aligned with global scientific standards.

This is not a short-term workshop. It is a long-term, publication-oriented research experience built for serious learners who aspire to build a strong foundation in computational cancer genomics.

Objective of the Project

The primary objective of this research project is:

• To model and analyze CRISPR-Cas9 perturbations at single-cell resolution

• To decode gene regulatory network (GRN) rewiring within the tumour microenvironment

• To connect genomic perturbations with cancer immunotherapy insights

• To develop publication-ready analytical frameworks

• To train participants in hypothesis-driven computational research

The focus is on scientific thinking, analytical rigor, and translational relevance.

Methodology

The project follows a structured, multi-phase research framework:

Phase 1: Data Acquisition & Preprocessing

• Collection and curation of single-cell RNA-seq datasets

• Quality control and normalization

• Dimensionality reduction and clustering

Phase 2: CRISPR Perturbation Modeling

• Analysis of CRISPR-Cas9 editing outcomes

• Differential gene expression analysis

• Integration of perturbation data with cellular heterogeneity

Phase 3: Gene Regulatory Network Inference

• GRN construction and regulatory rewiring analysis

• Pathway enrichment and functional annotation

• Biological interpretation aligned with immunotherapy relevance

Phase 4: Scientific Reporting & Publication Framework

• Visualization of findings

• Structured research documentation

• Manuscript-oriented workflow training

Participants will use globally accepted open-source tools widely cited in high-impact journals. All sessions combine theory + hands-on practice using various computational tools.